How does adjusting CI-110P image brightness affect LAI?

The effect of adjusting the brightness depends on whether it is done during image acquisition or afterwards, during image processing. If you have already taken the image and are now processing it afterwards, the following relationship is expected: LAI will decrease with increasing brightness. This is because plant elements, such as back-lit leaves or light green leaves, will be washed-out in the image and will appear as sky to the software. Increased brightness= more sky = decreased LAI.

To counter-act this, re-assign "Foliage" and "Sky" colors in the Sky and Leaf Filter option after setting the desired brightness, it is recommended to:

Adjust the brightness so the image appears properly exposed. (Go to the Threshold menu and choose a method. Adjust the slider until all the plant elements appear green. Press the Data button. Go back and adjust the brightness/contrast until all the plant elements are green. Keep adjusting the settings until you have grouped all the plant elements together and separated the sky or background)