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Calibrating the Legacy CI-710

Instructions to calibrate the Legacy CI-710

These instructions should be followed each time the CI-710 is used. Calibration is required before first using the CI-710. The instrument should also be calibrated when switching between modes. It is recommended to re-calibrate the CI-710 frequently when using it, especially in Reflectance Mode. For best results allow the CI-710 to warm-up for 15-30 minutes prior to calibration and measurements. The light sources in the CI-710 are affected by heat. Allowing time for warm-up reduces spectrum drift. The user cannot calibrate in scope mode but should first use this mode to ensure that the signal peaks are not oversaturated.

  1. Start SpectraSnap!
  2. Select the green Start button on the toolbar.
  3. Open the Setup menu.
  4. Click Mode and select Scope . This means the raw data from the spectrometer will be displayed.
  5. Place the reflectance standard in the leaf clip with the white circle facing the light. The light will either come from the top or bottom depending on where the fiber optic cable is connected (see Fig. 9 & 10).
  6. Select Setup and increase or decrease the Integration Time until the data displayed is fully on the graph (below 60,000 on the y-axis). This ensures maximum resolution for the entire spectrum. If the line appears to go higher than 60,000, decrease the integration time until the entire line appears on the graph.

    Note: If you are only inspecting a specific band, you could increase the Integration Time until that band reaches the ceiling of 60,000. If the integration time is set too high, an error message will appear after calibration indicating: “The integration time is causing the spectrometer to be over saturated at some wavelengths. Switch to Scope mode to see which areas are over-saturated. Lower the integration time to prevent this problem.”
  7. Change the Boxcar Width and Scans to Average as desired. 
  8. Select the Mode of operation for the CI-710.
  9. To calibrate for reflectance measurements, ensure that the reflectance standard is inserted with the white circle facing down and the optics connected to the side of the unit (rather than the leaf clip).
  10. To calibrate for transmission or absorbance measurements, ensure nothing is inserted into the leaf clip and that the optics is connected to the leaf clip.
  11. Select Calibrate Follow the on-screen prompts.